A Fully Integrated Cell-based Cytotoxicity, Gene and Protein Expression Analysis Platfrom
نویسندگان
چکیده
We demonstrate a novel, user-friendly biological microprocessor for highly efficient cell capture, culture, treatment and analysis. The device provides a multipurpose platform for a wide range of molecular and cell biology methods. As pilot application, we implement a cell-based cytotoxicity assay as well as gene and protein expression studies in both adherent and non-adherent mammalian cells. The gene expression studies are performed on mRNA level based on real-time NucleicAcid-Sequence Based Amplification (NASBA) while the protein expression analysis uses fluorescent immunostaining.
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